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Hesperidin synergistically potentiates the sleep-enhancing effect of diazepam in mice.

The study found that hesperidin, a natural flavonoid, synergistically interacts with diazepam to enhance sleep-related behavior as measured in the hole board test. Isobolar analysis confirmed the interaction was synergistic, not additive, and pharmacokinetic effects or direct binding of hesperidin to the benzodiazepine site were ruled out.

1 min readUpdated Jul 7, 20260 RCTsView structured evidence →
Evidence Score24/100
Human RCT☆☆☆☆☆
Meta-analysis☆☆☆☆☆
Mechanism★★★★★
Safety★★★★
Confidencelow

This article is automatically generated from the structured evidence profile behind the claim above. Scores reflect the quality and quantity of available research, not clinical advice.

Hesperidin synergistically potentiates the sleep-enhancing effect of diazepam in mice. The current body of evidence comprises 1 study. EvidenceHub rates the overall confidence at 24/100 (low).

The Claim

Hesperidin synergistically potentiates the sleep-enhancing effect of diazepam in mice.

This conclusion is most relevant to: Mice (animal study).

What the Research Shows

The conclusion draws on 1 linked study. Highlights from the cited literature:

  • Synergistic interaction between hesperidin, a natural flavonoid, and diazepam. (European journal of pharmacology, 2005) —

How It Works

The proposed biological pathway:

  • Hesperidin does not bind to the benzodiazepine site on GABA(A) receptors
  • Diazepam acts as an agonist at the benzodiazepine binding site
  • Co-administration produces synergistic enhancement of sleep effects
  • Result: Potentiation of sleep-enhancing properties

Who Might Benefit

Evidence fit by population:

  • Mice (animal study)

Limitations & Caveats

Important context when interpreting this evidence:

  • Animal study, not directly generalizable to humans
  • No specific dose-response data or effect sizes reported in abstract

Frequently Asked Questions

What is hesperidin?

Hesperidin is a natural flavonoid found in Valeriana and citrus fruits, with sedative and sleep-enhancing properties.

How does hesperidin interact with diazepam?

Hesperidin synergistically potentiates the sleep-enhancing effect of diazepam, meaning the combined effect is greater than the sum of individual effects.

Does hesperidin bind to benzodiazepine receptors?

No, hesperidin is not a ligand for the benzodiazepine binding site on GABA(A) receptors.

Could hesperidin reduce the needed dose of benzodiazepines?

The authors suggest that hesperidin may allow for lower therapeutic doses of benzodiazepines due to synergistic interaction.

References

  1. 1.Fernández SP, Wasowski C, Paladini AC, Marder M. “Synergistic interaction between hesperidin, a natural flavonoid, and diazepam..” European journal of pharmacology, 2005. PMID: 15840404 DOI: 10.1016/j.ejphar.2005.02.039
Disclaimer: This article is auto-generated from structured research data for educational purposes only and is not medical advice. Evidence scores reflect the quality and quantity of available research, not clinical recommendations. Always consult a healthcare professional before starting any supplement or intervention.