Lactobacillus reuteri E9 regulates sleep disorders by modulating inhibitory neurotransmitters and sleep-related receptor expression via the microbiota-gut-brain axis.
In a PTZ-induced zebrafish model of sleep disorder, Lactobacillus reuteri E9 significantly upregulated inhibitory neurotransmitters including GABA, taurine, glycine, and serine (p < 0.05). It also reduced seizures and hyperactivity, and upregulated GABA receptor genes and melatonin receptor (Mtnr1aa) genes in neural tissue.
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Lactobacillus reuteri E9 regulates sleep disorders by modulating inhibitory neurotransmitters and sleep-related receptor expression via the microbiota-gut-brain axis. The current body of evidence comprises 1 study. EvidenceHub rates the overall confidence at 24/100 (low).
The Claim
Lactobacillus reuteri E9 regulates sleep disorders by modulating inhibitory neurotransmitters and sleep-related receptor expression via the microbiota-gut-brain axis.
This conclusion is most relevant to: Pentylenetetrazol (PTZ)-induced zebrafish model of sleep disorder.
What the Research Shows
The conclusion draws on 1 linked study. Highlights from the cited literature:
- ▸Lactobacillus reuteri E9 Regulates Sleep Disorders Through Its Metabolite GABA. (Frontiers in bioscience (Landmark edition), 2025) —
How It Works
The proposed biological pathway:
- ▸Lactobacillus reuteri E9 is administered to PTZ-induced zebrafish
- ▸E9 upregulates inhibitory neurotransmitters (GABA, taurine, glycine, serine)
- ▸E9 upregulates GABA receptor and melatonin receptor (Mtnr1aa) gene expression in neural tissue
- ▸Result: Reduced seizures and hyperactivity, indicating sedative and sleep-regulating effects
Who Might Benefit
Evidence fit by population:
- ▸Pentylenetetrazol (PTZ)-induced zebrafish model of sleep disorder
Recommended Dose
N/A
Limitations & Caveats
Important context when interpreting this evidence:
- ▸Animal model (zebrafish) may not fully translate to human sleep disorders
- ▸Abstract does not report effect sizes or confidence intervals for key outcomes
Frequently Asked Questions
What is Lactobacillus reuteri E9?▼
It is a non-toxic, drug-resistant probiotic strain isolated and characterized for its potential psychobiotic effects on sleep.
How does Lactobacillus reuteri E9 affect sleep?▼
It upregulates inhibitory neurotransmitters like GABA and taurine, and increases expression of GABA and melatonin receptors, leading to sedative effects in a zebrafish model.
What was the study model used?▼
The study used a pentylenetetrazol (PTZ)-induced zebrafish model of sleep disorder to evaluate the effects of E9.
Is Lactobacillus reuteri E9 safe for humans?▼
The abstract states it is non-toxic and drug-resistant, but human safety and efficacy have not been tested in this study.
References
- 1.Jiang Y, Guo L, He H, Chen H, Chen T, Liu Y, Zhao W. “Lactobacillus reuteri E9 Regulates Sleep Disorders Through Its Metabolite GABA..” Frontiers in bioscience (Landmark edition), 2025. PMID: 40613302 DOI: 10.31083/FBL39587