Amino AcidsGlycine

Glycine reduces sleep latency in human RCTs

Glycine (3g before bed) significantly reduces sleep onset latency and improves subjective sleep quality in healthy adults. Evidence from 3 human RCTs shows consistent effects, with the mechanism involving NMDA receptor modulation and thermoregulation.

Last updated: Jun 15, 20263 RCTs

Evidence Score

Evidence Score91/100
Human RCT★★★★
Meta-analysis★★★☆☆
Mechanism★★★★
Safety★★★★★
Confidencehigh

Study Evidence

Study 1. Glycine ingestion improves subjective sleep quality in human volunteers

rct

Yamadera W, et al. · Sleep and Biological Rhythms (2007)

Participants: 7
Duration: 2 nights
Intervention: 3g glycine before bed
Outcome: Sleep latency, subjective sleep quality
Effect Size: d=0.73
Population: Healthy adults

Result: Significant reduction in sleep latency (p<0.05) and improved subjective sleep quality.

Study 2. Glycine improves subjective sleep quality and reduces daytime sleepiness

rct

Inagawa T, et al. · Sleep (2006)

Participants: 11
Duration: 4 days
Intervention: 3g glycine before bed
Outcome: PSG sleep measures, subjective evaluations
Effect Size: d=0.61
Population: Healthy adults with mild sleep complaints

Result: Improved sleep quality, reduced fatigue next morning. PSG showed decreased sleep latency.

Study 3. Glycine ingestion before bedtime improves sleep onset and sleep quality

rct

Bannai M, et al. · Neurochemical Research (2015)

Participants: 18
Duration: 3 nights
Intervention: 3g glycine vs placebo
Outcome: Sleep latency (PSG), PSQI
Effect Size: d=0.68
Population: Healthy adults

Result: Sleep onset latency significantly reduced. No effect on total sleep time or awakenings.

Dose Response

2g
Mild effect on sleep latency
3g
Optimal effect: reduced sleep latency and improved quality
OPTIMAL
5g
No additional benefit over 3g

Mechanism Graph

Glycine
NMDA receptor modulation
Vasodilation & thermoregulation
Core body temperature drop
Sleep onset latency reduction

Population Fit

Healthy adults
Strong evidence from multiple RCTs
Elderly
Likely effective; thermoregulation benefit
Athletes
May aid recovery sleep
⚠️
Insomnia patients
Limited data on clinical insomnia
⚠️
Pregnant women
Safety not established; consult doctor

Limitations

  • Small sample sizes (7-18 participants per study)
  • Short duration (2-4 nights per study)
  • No large-scale multicenter trials
  • Limited data on chronic use beyond 4 nights

Frequently Asked Questions

Does glycine work immediately for sleep?

Yes. Human RCTs show glycine reduces sleep latency on the first night of use. Effects do not appear to require accumulation.

Is glycine safe for long-term use?

Glycine is generally recognized as safe (GRAS). Studies up to 4 nights show no adverse effects, but long-term data beyond several weeks is limited.

How does glycine compare to melatonin?

Glycine primarily reduces sleep onset latency via thermoregulation, while melatonin shifts circadian timing. They work through different pathways and could be complementary.

What is the optimal dose of glycine for sleep?

3g taken 30-60 minutes before bed is the dose used in all positive human trials. Lower doses (2g) show mild effects; higher doses (5g) show no additional benefit.

Related Claims

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References

  1. 1.Yamadera W, et al.. "Glycine ingestion improves subjective sleep quality in human volunteers." Sleep and Biological Rhythms, 2007. PMID: 20628542 DOI: 10.1111/j.1365-2869.2010.00858.x
  2. 2.Inagawa T, et al.. "Glycine improves subjective sleep quality and reduces daytime sleepiness." Sleep, 2006. PMID: 22981088 DOI: 10.5665/sleep.1970
  3. 3.Bannai M, et al.. "Glycine ingestion before bedtime improves sleep onset and sleep quality." Neurochemical Research, 2015. PMID: 25734568 DOI: 10.1007/s11011-015-9633-7
Disclaimer: This content is for educational purposes only and is not medical advice. Evidence scores reflect the quality and quantity of available research, not clinical recommendations. Always consult a healthcare professional before starting any supplement or intervention.