Hormones · Melatonin

Co-administration of cannabidiol and L-theanine synergistically improves sleep-related outcomes in a caffeine-induced sleep disturbance mouse model

In a mouse model of caffeine-induced sleep disturbance, combined administration of CBD and L-theanine at ratios of 1:1, 2:1, or 1:2 significantly reduced sleep onset latency and increased total sleep duration compared to single treatments. Combination index analysis indicated a synergistic interaction at lower doses, and combination treatment was associated with over 40% increases in serum serotonin and melatonin levels, as well as elevated cortical GABA levels.

1 min read•Updated Jul 7, 2026•0 RCTs•View structured evidence →
Evidence Score26/100
Human RCT☆☆☆☆☆
Meta-analysis☆☆☆☆☆
Mechanism☆☆☆☆☆
Safety☆☆☆☆☆
Confidencelow

This article is automatically generated from the structured evidence profile behind the claim above. Scores reflect the quality and quantity of available research, not clinical advice.

Co-administration of cannabidiol and L-theanine synergistically improves sleep-related outcomes in a caffeine-induced sleep disturbance mouse model The current body of evidence comprises 1 study. EvidenceHub rates the overall confidence at 26/100 (low).

The Claim

Co-administration of cannabidiol and L-theanine synergistically improves sleep-related outcomes in a caffeine-induced sleep disturbance mouse model

This conclusion is most relevant to: Caffeine-induced sleep disturbance mouse model.

What the Research Shows

The conclusion draws on 1 linked study. Highlights from the cited literature:

  • ▸Combined Administration of Cannabidiol and L-Theanine Improves Sleep-Related Outcomes in Caffeine-Induced Sleep Disturbances Mouse Model. (Biomolecules & therapeutics, 2026) —

How It Works

The proposed biological pathway:

  • ▸CBD increases serotonin levels in a dose-dependent manner
  • ▸L-theanine elevates serotonin at lower doses
  • ▸Combination treatment elevates serum serotonin and melatonin by >40%
  • ▸Combination treatment elevates cortical GABA levels
  • ▸Result: Improved sleep onset latency and total sleep duration

Who Might Benefit

Evidence fit by population:

  • ▸Caffeine-induced sleep disturbance mouse model

Limitations & Caveats

Important context when interpreting this evidence:

  • ▸Animal model (mice) limits direct translation to humans
  • ▸Precise mechanisms remain to be determined

References

  1. 1.Kim J, Yu YJ, Kim HK, Ullah A, Bappi MH, Shim HJ, Cheong J, Compomayor NB, Kim HJ, Chae HJ. “Combined Administration of Cannabidiol and L-Theanine Improves Sleep-Related Outcomes in Caffeine-Induced Sleep Disturbances Mouse Model..” Biomolecules & therapeutics, 2026. PMID: 42375087 DOI: 10.4062/biomolther.2025.160
Disclaimer: This article is auto-generated from structured research data for educational purposes only and is not medical advice. Evidence scores reflect the quality and quantity of available research, not clinical recommendations. Always consult a healthcare professional before starting any supplement or intervention.