Co-administration of cannabidiol and L-theanine synergistically improves sleep-related outcomes in a caffeine-induced sleep disturbance mouse model

In a mouse model of caffeine-induced sleep disturbance, combined administration of CBD and L-theanine at ratios of 1:1, 2:1, or 1:2 significantly reduced sleep onset latency and increased total sleep duration compared to single treatments. Combination index analysis indicated a synergistic interaction at lower doses, and combination treatment was associated with over 40% increases in serum serotonin and melatonin levels, as well as elevated cortical GABA levels.