L-theanine prevents myocardial injury in sleep-deprived mice by suppressing ferroptosis through SIRT1
L-theanine alleviated sleep deprivation-induced tachycardia, restored myocardial and mitochondrial integrity, and reduced oxidative damage markers including ROS and Fe2+ in H9c2 cells. The protective effect was counteracted by the SIRT1 inhibitor EX-527, indicating that L-theanine mitigates cardiac injury primarily by suppressing ferroptosis through SIRT1 in cardiomyocytes.
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L-theanine prevents myocardial injury in sleep-deprived mice by suppressing ferroptosis through SIRT1 The current body of evidence comprises 1 study. EvidenceHub rates the overall confidence at 24/100 (low).
The Claim
L-theanine prevents myocardial injury in sleep-deprived mice by suppressing ferroptosis through SIRT1
This conclusion is most relevant to: Sleep-deprived mice (C57BL/6) and H9c2 rat cardiomyocyte cell line.
What the Research Shows
The conclusion draws on 1 linked study. Highlights from the cited literature:
- ▸L-theanine prevents myocardial injury in sleep‑deprived mice by suppressing ferroptosis through SIRT1. (Naunyn-Schmiedeberg's archives of pharmacology, 2025) —
How It Works
The proposed biological pathway:
- ▸Sleep deprivation induces oxidative stress and ferroptosis in cardiomyocytes
- ▸L-theanine upregulates SIRT1 expression
- ▸SIRT1 suppresses ferroptosis-related protein expression and reduces ROS and Fe2+ levels
- ▸Result: Prevention of myocardial injury and restoration of cardiac function
Who Might Benefit
Evidence fit by population:
- ▸Sleep-deprived mice (C57BL/6) and H9c2 rat cardiomyocyte cell line
Recommended Dose
N/A
Limitations & Caveats
Important context when interpreting this evidence:
- ▸Animal model and in vitro cell line may not fully replicate human physiology
- ▸Specific dosage and administration route of L-theanine not reported in abstract
Frequently Asked Questions
What is the primary mechanism by which L-theanine protects the heart in sleep-deprived mice?▼
L-theanine suppresses ferroptosis through upregulation of SIRT1 in cardiomyocytes.
Did the study use human participants?▼
No, the study used a mouse model of sleep deprivation and H9c2 rat cardiomyocyte cells.
What evidence supports the role of SIRT1 in L-theanine's effect?▼
The SIRT1 inhibitor EX-527 counteracted the protective effect of L-theanine against ferroptosis, confirming SIRT1's involvement.
What markers of cardiac injury were measured?▼
Cardiac function parameters, myocardial and mitochondrial integrity, oxidative stress markers (ROS, Fe2+), and ferroptosis-related protein expression.
References
- 1.Huang X, Lu X, Wu Y, Wu Z, Li M, Miao Y, Xie Z, Gong Z, Cao Y. “L-theanine prevents myocardial injury in sleep‑deprived mice by suppressing ferroptosis through SIRT1..” Naunyn-Schmiedeberg's archives of pharmacology, 2025. PMID: 40328913 DOI: 10.1007/s00210-025-04206-8