Amino AcidsL-Theanine

L-theanine prevents myocardial injury in sleep-deprived mice by suppressing ferroptosis through SIRT1

L-theanine alleviated sleep deprivation-induced tachycardia, restored myocardial and mitochondrial integrity, and reduced oxidative damage markers including ROS and Fe2+ in H9c2 cells. The protective effect was counteracted by the SIRT1 inhibitor EX-527, indicating that L-theanine mitigates cardiac injury primarily by suppressing ferroptosis through SIRT1 in cardiomyocytes.

Last updated: Jul 8, 2026•0 RCTs•📖 Read as article →

Evidence Score

Evidence Score24/100
Human RCT☆☆☆☆☆
Meta-analysis☆☆☆☆☆
Mechanism★★★★★
Safety★★★★☆
Confidencelow

Study Evidence

Study 1. L-theanine prevents myocardial injury in sleep‑deprived mice by suppressing ferroptosis through SIRT1.

observational

Huang X, Lu X, Wu Y, Wu Z, Li M, Miao Y, Xie Z, Gong Z, Cao Y · Naunyn-Schmiedeberg's archives of pharmacology (2025)

Participants: N/A
Duration: N/A
Intervention: L-theanine administration in sleep-deprived mice and erastin-treated H9c2 cells
Outcome: Cardiac function parameters, myocardial and mitochondrial integrity, oxidative stress markers (ROS, Fe2+), SIRT1 and ferroptosis-related protein expression
Effect Size: N/A
Population: Sleep-deprived mice (C57BL/6) and H9c2 rat cardiomyocyte cell line

Result:

PubMed: 40328913DOI: 10.1007/s00210-025-04206-8

Mechanism Graph

Sleep deprivation induces oxidative stress and ferroptosis in cardiomyocytes
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L-theanine upregulates SIRT1 expression
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SIRT1 suppresses ferroptosis-related protein expression and reduces ROS and Fe2+ levels
↓
Result: Prevention of myocardial injury and restoration of cardiac function

Limitations

  • ⚠Animal model and in vitro cell line may not fully replicate human physiology
  • ⚠Specific dosage and administration route of L-theanine not reported in abstract

Frequently Asked Questions

What is the primary mechanism by which L-theanine protects the heart in sleep-deprived mice?▼

L-theanine suppresses ferroptosis through upregulation of SIRT1 in cardiomyocytes.

Did the study use human participants?▼

No, the study used a mouse model of sleep deprivation and H9c2 rat cardiomyocyte cells.

What evidence supports the role of SIRT1 in L-theanine's effect?▼

The SIRT1 inhibitor EX-527 counteracted the protective effect of L-theanine against ferroptosis, confirming SIRT1's involvement.

What markers of cardiac injury were measured?▼

Cardiac function parameters, myocardial and mitochondrial integrity, oxidative stress markers (ROS, Fe2+), and ferroptosis-related protein expression.

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References

  1. 1.Huang X, Lu X, Wu Y, Wu Z, Li M, Miao Y, Xie Z, Gong Z, Cao Y. "L-theanine prevents myocardial injury in sleep‑deprived mice by suppressing ferroptosis through SIRT1.." Naunyn-Schmiedeberg's archives of pharmacology, 2025. PMID: 40328913 DOI: 10.1007/s00210-025-04206-8
Disclaimer: This content is for educational purposes only and is not medical advice. Evidence scores reflect the quality and quantity of available research, not clinical recommendations. Always consult a healthcare professional before starting any supplement or intervention.