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Conserved sleep orthologs, including DnaK, SHMT, and potassium channel proteins, are present in Epsilonproteobacteria and show sequence similarities of 39.13% to 61.45%.

Phylogenomic analysis of Sulfurimonas paralvinellae identified conserved sleep-related orthologs such as DnaK (Hsp70), serine hydroxymethyltransferase, and potassium channel family proteins, with sequence similarities ranging from 39.13% to 61.45%. Proteins with fewer domains, like adenylate kinase, showed greater conservation, while bifunctional protein-serine/threonine kinases and phosphatases exhibited adaptations linked to the extremophilic environment. Gene Ontology analysis highlighted catalytic activity, potassium channel function, and cellular processes, emphasizing ion channels in sleep-wake regulation.

Last updated: Jul 7, 2026•0 RCTs•📖 Read as article →

Evidence Score

Evidence Score32/100
Human RCT☆☆☆☆☆
Meta-analysis☆☆☆☆☆
Mechanism★★★★★
Safety★★★★☆
Confidencelow

Study Evidence

Study 1. Studying sleep orthologs in Epsilonproteobacteria through an evolutionary lens: investigating sleep mysteries through phylogenomics.

observational

Pandi-Perumal SR, Saravanan KM, Paul S, Warren Spence D, Chidambaram SB · World journal of microbiology & biotechnology (2025)

Participants: N/A
Duration: N/A
Intervention: Phylogenomic analysis of sleep-related genes in Sulfurimonas paralvinellae
Outcome: Identification and sequence similarity of conserved sleep orthologs (DnaK, SHMT, potassium channel proteins); Gene Ontology categories (catalytic activity, potassium channel function, cellular processes)
Effect Size: N/A
Population: Sulfurimonas paralvinellae (Epsilonproteobacteria class, extremophilic organism)

Result:

PubMed: 40289222DOI: 10.1007/s11274-025-04361-3

Mechanism Graph

Phylogenomic methods applied to identify sleep-related orthologs in Epsilonproteobacteria
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Conserved orthologs found: DnaK, SHMT, potassium channel proteins (39.13%-61.45% similarity)
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Proteins with fewer domains (e.g., adenylate kinase) showed higher conservation
↓
Bifunctional kinases/phosphatases adapted to high-pressure/high-temperature conditions
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Gene Ontology analysis linked potassium channels to sleep-wake cycle regulation

Limitations

  • ⚠Study is based on computational phylogenomics and sequence analysis, not experimental validation
  • ⚠Findings are limited to a single extremophilic species (S. paralvinellae), reducing generalizability
  • ⚠No direct behavioral or physiological sleep measurements were taken

Frequently Asked Questions

What sleep-related genes were found in Epsilonproteobacteria?▼

Conserved orthologs include DnaK (Hsp70), serine hydroxymethyltransferase (SHMT), and potassium channel family proteins.

How similar are these sleep orthologs to known sleep genes?▼

Sequence similarities range from 39.13% to 61.45%.

Why are potassium channels important in this study?▼

Gene Ontology analysis indicated potassium channel function is significant for regulating the sleep-wake cycle.

What is the significance of studying sleep genes in bacteria?▼

It provides an evolutionary perspective on sleep-related genes and their association with metabolic and environmental changes.

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References

  1. 1.Pandi-Perumal SR, Saravanan KM, Paul S, Warren Spence D, Chidambaram SB. "Studying sleep orthologs in Epsilonproteobacteria through an evolutionary lens: investigating sleep mysteries through phylogenomics.." World journal of microbiology & biotechnology, 2025. PMID: 40289222 DOI: 10.1007/s11274-025-04361-3
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