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Flavonoids inhibit MCT1-mediated transport of GHB, reducing sleep time in rats

In vitro, flavonoids such as luteolin, morin, and phloretin inhibited MCT1-mediated uptake of GHB in rat MCT1-transfected cells, with IC50 values of 0.41, 6.41, and 2.57 μM, respectively. In rats, intravenous luteolin (10 mg/kg) increased renal and total clearance of GHB and significantly decreased sleep time from 165 ± 10 min to 121 ± 5 min after a 1000 mg/kg GHB dose.

Last updated: Jul 7, 20260 RCTs📖 Read as article →

Evidence Score

Evidence Score26/100
Human RCT☆☆☆☆☆
Meta-analysis☆☆☆☆☆
Mechanism☆☆☆☆☆
Safety☆☆☆☆☆
Confidencelow

Study Evidence

Study 1. Flavonoids modulate monocarboxylate transporter-1-mediated transport of gamma-hydroxybutyrate in vitro and in vivo.

observational

Wang Q, Morris ME · Drug metabolism and disposition: the biological fate of chemicals (2007)

Participants: N/A
Duration: Acute (single dose)
Intervention: Intravenous administration of luteolin (10 mg/kg) with or without GHB (1000 mg/kg) in rats
Outcome: Sleep time, renal clearance, total clearance of GHB
Effect Size: Sleep time reduction: 44 minutes (121 ± 5 vs 165 ± 10 min)
Population: Male Sprague-Dawley rats

Result:

Mechanism Graph

Flavonoids bind to MCT1 transporter
Competitive inhibition of GHB uptake at MCT1
Reduced renal reabsorption of GHB
Increased GHB clearance and decreased sleep time

Limitations

  • Animal model (rats) may not directly translate to human physiology
  • Only one flavonoid (luteolin) tested in vivo; effects of other flavonoids not confirmed in living animals

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References

  1. 1.Wang Q, Morris ME. "Flavonoids modulate monocarboxylate transporter-1-mediated transport of gamma-hydroxybutyrate in vitro and in vivo.." Drug metabolism and disposition: the biological fate of chemicals, 2007. PMID: 17108059 DOI: 10.1124/dmd.106.012369
Disclaimer: This content is for educational purposes only and is not medical advice. Evidence scores reflect the quality and quantity of available research, not clinical recommendations. Always consult a healthcare professional before starting any supplement or intervention.